Position Profile
Biologics Research Center (BRC) is responsible to create new Biologics entities ranging from monoclonal antibodies to AAV molecules, and to ensure their fitness to requirements that a drug must fulfill. Integrity determination is an important aspect for the characterization of biologics, especially for new formats and modalities. Currently in-vitro and in-vivo assessments are mostly done using Elisa type of assays or by LC-MS/MS detection of specific marker peptides. These technologies provide mainly identity readout (with quantitation) but not any results on the integrity of the molecule. For this purpose, immune purification followed by intact mass analyses must be performed. It is currently already existing but often lacks the sensitivity for following the in-vivo (e.g., mouse) fate of a molecule over time.
The goal of this project is to set up a sensitive immuno-purification (IP) followed by μLC-MS integrity determination either by rpLC-MS or native SEC-MS.
Responsibilities will include but are not limited to:
Set-up sensitive immuno-purification workflow on various antibodies, proteins, and related molecules.
Set-up sensitive analyses by mass spectrometry (rpLC-MS and/or Native SEC-MS) of the molecules which have been immune-purified.
Processing of data, result interpretation and preparation of summary documents and reports.
Writing of laboratory protocols
What you’ll bring to the role:
Education: MSc or equivalent education in Analytical chemistry, Biochemistry, Biotechnology or a related discipline.
Experience:
Practical experience to the characterization proteins, antibodies, and related molecules, especially with mass spectrometry would be of benefit. Solid theoretical knowledge of protein chemistry and analytical methods for characterization of proteins, e.g., liquid chromatography would be of advantage.
Diligent and accurate working style, as well as communication and organizational skills are required.
Please contact : thierry.besson@novartis.com
Description
